Unravelling Epigenetic changes in Diabetic Cardiomyopathy: A Novel Approach Using Human iPSC-Derived Cardiac Organoids

This exciting cross-translational project between Coventry University and University Hospitals Coventry & Warwickshire will explore epigenetic alterations that occur in the diabetic heart. Coventry University is inviting applications from suitably-qualified graduates for a (fully-funded) PhD studentship based in Centre For Health and Life Sciences.

Project details

This exciting fully funded PhD studentship offers an opportunity to explore the correlation between Type 2 Diabetes (T2D) and increased mortality from myocardial infarctions (MI) in 3D cell culture models. People living with T2D have a twofold risk of dying from heart disease, accounting for 30-50% of all deaths in this group (BHF). Key factors in this increased mortality post-MI are elevated long-chain fatty acids (LCFAs) and failure to adapt to hypoxia (Dodd et al 2018).

The project aims to create a cardiac organoid model to focus on the role of alpha-ketoglutarate dependent oxygenase in controlling hypoxic adaptation processes, including RNA/DNA/histone methylation. Understanding the control of these adaptations and the molecular mechanisms at play is crucial for developing novel therapeutics to increase post-MI survival.

Under the guidance of Dr Michael Dodd, Professor Helen Maddock (Coventry University), and Professor Faizel Osman (University Hospitals Coventry & Warwickshire (UHCW)), the project aims to generate a human induced pluripotent stem cell (hIPSC) cardiac organoid model of diabetic cardiomyopathy. The model will be used to study how hypoxia and diabetes alter the epigenetic profile of 3D hiPSC cardiac organoids. With UHCW this model will be validated using cardiac tissue from T2D and control patients.

Additional requirements

• Knowledge and experience in cardiovascular metabolism
• Knowledge in cardiovascular epigenetic modification
• Knowledge of type 2 diabetes
• Experience of mammalian cell culture techniques, including stem cells

Funding

Tuition fees and bursary

Benefits

The successful candidate will receive comprehensive research training including technical, personal and professional skills. All researchers at Coventry University (from PhD to Professor) are part of the Doctoral and Research College and Centre which provides support with high-quality training and career development activities.

Entry requirements

• A minimum of a 2:1 first degree in a relevant discipline/subject area with a minimum 60% mark in the project element or equivalent with a minimum 60% overall module average.

PLUS

• The potential to engage in innovative research and to complete the PhD within 3.5 years.
• A minimum of English language proficiency (IELTS academic overall minimum score of 7.0 with a minimum of 6.5 in each component).

How to apply

To find out more about the project, please contact Professor ac8844@coventry.ac.uk

All applications require full supporting documentation, a covering letter, plus a 2000-word supporting statement showing how the applicant’s expertise and interests are relevant to the project.