Postdoctoral Research Associate – Biophysics Core

Details

Posted: 15-Oct-23

Location: Chicago, Illinois

Type: Full-time

Salary: Open

Categories:

Academic/Faculty
Biological/Biomedical Sciences

Internal Number: 4693712

Postdoctoral Research Associate – Biophysics Core
Postdoctoral Research Associate – Biophysics Core
Hiring Department: OVCR – RRC Biophysics
Location: Chicago, IL USA
Requisition ID: 1021747
Posting Close Date: 10/27/2023
About the University of Illinois Chicago
UIC is among the nation’s preeminent urban public research universities, a Carnegie RU/VH research institution, and the largest university in Chicago. UIC serves over 34,000 students, comprising one of the most diverse student bodies in the nation and is designated as a Minority Serving Institution (MSI), an Asian American and Native American Pacific Islander Serving Institution (AANAPSI) and a Hispanic Serving Institution (HSI). Through its 16 colleges, UIC produces nationally and internationally recognized multidisciplinary academic programs in concert with civic, corporate and community partners worldwide, including a full complement of health sciences colleges. By emphasizing cutting-edge and transformational research along with a commitment to the success of all students, UIC embodies the dynamic, vibrant and engaged urban university. Recent ‘Best Colleges’ rankings published by U.S. News & World Report, found UIC climbed up in its rankings among top public schools in the nation and among all national universities. UIC has nearly 260,000 alumni, and is one of the largest employers in the city of Chicago.
Description:
Position Summary
Perform research in Under supervision of the Biophysics Core Director, the Postdoctoral Research Associate plans and conducts experiments in various protein over-expression and purification, binding analyses of biomolecules, biological or biochemical assays, and structural studies using X-ray crystallography and Cryo-EM. The work involves the application of lab specific tests and developing new protocols, and train users and students in various techniques and research equipment. This position requires abilities not only to optimize/trouble-shoot existing protocols, but also to create new protocols independently in order to make each assay work properly.
There are many projects on going, three of which are below.
1. Target-based anti-tuberculosis (TB) drug developments.
2. Antimicrobial drug development against three coronaviruses (SARS-CoV-1, SARS-CoV-2, and MERS-CoV).
3. Identification of a new role of membrane-type 1 matrix metalloproteinases in corneal neovascularization.
4. Development of MMP14-laden exosomes as a novel anti-SARS-CoV-2 therapy
Duties & Responsibilities

Perform plasmid transformation into E. coli cells and small and large-scale bacterial culture and protein over-expression. Optimize over-expression condition for each protein.

Manage and perform various protein purification using affinity, ion-exchange and size-exclusion chromatography using AKTA FPLCs. Optimize purification conditions for each protein and do quality control (QC).

Create enzyme assay protocol independently to analyze each enzyme activity and run Fluorescence- or luminescence-based enzymatic assay with various enzymes using microplate reader.

Prepare crystal samples, set up crystals, and check crystal growth. Diffract crystals at X-ray beamline and solve structures if possible.

Optimize conditions for Cryo-EM samples and solve protein structures if possible.

Trouble-shoot and optimize existing protocols independently to make assays work upon encountering problems. Conduct literature searches for technology updates and establish new protocols.

Train students and postdocs who want to use Biophysics core instruments, which require a wide range of knowledge and expertise for various biophysical instruments such as Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Mass Photometry (MP), and Circular dichroism (CD).

Manage weekly and monthly maintenance procedures on core instruments (AKTA Pure & AKTAxpress FPLCs, Biacore T200, Biacore 8K, MicroCal PEAQ-ITC, Jasco 815 CD, Refeyn Mass Photometer, and Emulslflex C5.

Document all sample preparation, method development notes, and data analysis results in Excel and PowerPoint for publication and reports.

Perform other duties as assigned.

Perform other related duties and participate in special projects as assigned.

Qualifications:
Minimum Qualifications

• PhD degree in biochemistry, molecular biology, biophysics, structural biology or related discipline required.

Preferred Qualifications

• PhD with one or more of X-ray Crystallography (highly preferred), Cryo-EM (highly preferred), Nuclear Magnetic Resonance (NMR), Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), and Circular dichroism (CD) experiences are preferred.
• Protein purification using AKTA FPLC experiences preferred.

The University of Illinois System is an equal opportunity employer, including but not limited to disability and/or veteran status, and complies with all applicable state and federal employment mandates. Please visit Required Employment Notices and Posters to view our non-discrimination statement and find additional information about required background checks, sexual harassment/misconduct disclosures, COVID-19 vaccination requirement, and employment eligibility review through E-Verify.
The university provides accommodations to applicants and employees. Request an Accommodation
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The University of Illinois System is an equal opportunity employer, including but not limited to disability and/or veteran status, and complies with all applicable state and federal employment mandates. Please visit Required Employment Notices and Posters to view our non-discrimination statement and find additional information about required background checks, sexual harassment/misconduct disclosures, COVID-19 vaccination requirement, and employment eligibility review through E-Verify.
To apply, visit https://uic.csod.com/ux/ats/careersite/1/home/requisition/7281?c=uic
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About University of Illinois – Chicago
The University of Illinois Chicago is the city’s largest university and its only public Carnegie Research 1 institution. Its 16 academic colleges serve close to 34,000 undergraduate, graduate and professional students. UIC is recognized as one of the best public universities and one of the most ethnically rich and culturally diverse campuses in the nation, located in the heart of Chicago, it is an integral part of the city’s educational, technological and cultural fabric.
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