Postdoctoral Fellow Position in the Jeff Dodd-o Laboratory (Anesthesiology and Critical Care Medicine)

Johns Hopkins University School of Medicine
Baltimore, Maryland, US
Posted: 25 July 2022

Are you interested in research that is directly influenced by clinically important questions?
A postdoctoral position is available immediately, in the Jeff Dodd-o Laboratory in the Department of Anesthesiology and Critical Care Medicine at the Johns Hopkins University School of Medicine. We work closely with the Division of Pulmonary and Critical Care Medicine, as well as with transplant surgeons, to better understand the immunology of transplant rejection. Our overarching goal is to improve upon the efficacy of immunosuppression while reducing the debilitating side effects. The position will provide exposure and mentorship in the evaluation of immunoregulation through the use of lung injury and transplant models, flow cytometry, RNA and protein analysis

Job description:
A full-time Research Specialist position is available immediately in the laboratory of Jeffrey M Dodd-o, MD, PhD (https://anesthesiology.hopkinsmedicine.org/ ) in the Department of Anesthesiology and Critical Care Medicine at Johns Hopkins University. Our group studies the immunology of lung transplantation using both a novel murine lung ischemia:reperfusion model developed in the lab, as well as a murine, in-situ lung transplant model. We study the innate and adaptive immunity mechanisms leading to transplant rejection, with the goal of interrupting or preventing rejection completely. We have developed pharmacologic and chimeric methods, with our goal being a better understanding of the complete mechanistic pathways responsible for their success. The technology we use includes flow cytometry, CT scan, single cell RNA, and adoptive cell transfer.

Key requirements:
The candidate will conduct impactful physiologic experiments in a preclinical Murine model. All protocols follow AALAC guidelines. Techniques include delivering anesthesia, performing tracheal intubation, harvesting transplanted lungs, creating single cell suspensions, running and interpreting flow cytometry, western blots and immunoprecipitation, and biochemical assays (spectrophotometric, fluorogenic, luminescence). Cell harvesting for adoptive transfer, cell culturing, and developing new panels as well as assays of cytotoxic cell function are also part of our research protocol.

The responsibilities will include working alone as well as with collaborating research teams to conduct the murine ischemia;reperfusion surgeries, post-operative care of transplanted lung recipients, lung/spleen/bone marrow harvesting for the creation of single cell suspensions to be used in flow cytometry, adoptive cell transfer and cell culturing. You will work with the PI and collaborators to create new protocols evaluating cell cytokine production as well as panels for flow cytometry. Additional duties include running flow cytometry, its data entry and analysis, as well as animal feeding, cage clean-up and sanitation, and general laboratory organization.

Good oral communication skills in English and a collaborative and cooperative team spirit are required. Team work is essential in our laboratory. High priority candidates need to have, at a minimum, experience with in vitro work with human or animal cells and/or flow cytometry. Applicants with interest in immunology are encouraged to apply. An MD and/or PhD degree is required. Other helpful experience would be in cell culturing, immunohistochemistry, protein and DNA methods, microscopy (conventional and confocal), cell counting skills, and data processing.

The work schedule is flexible, although collaborations are often best performed between 9 a.m. and 5 p.m.. Only very infrequent weekend stop-ins for animal care will be requested.

Employment at Johns Hopkins University:
The employment is a 2-year position with a possibility of prolongation. A 6-month trial period will be applied. Postdoctoral fellow salary will be based on NIH guideline, individual health insurance [through the School of Medicine Student Health Program (SHP)] and dental insurance will be provided. Salary is commensurate with skills and experience and benefits will be provided.

Apply:
Interested candidates should submit a single document containing: 1) an updated CV (including date of the thesis defense, title of the thesis, previous academic/work positions, academic title, current position, academic distinctions and list of peer-reviewed publications), 2) a brief personal statement describing research interests and career goals, and 3) contact information for three references. Select applicants will be invited to interview. Please directly email the document to:

Dr. Jeffrey M Dodd-o, M.D., PhD
jdoddo@jhmi.edu
Associate Professor, Department of Anesthesiology
Department of Anesthesiology and Critical Care Medicine
Johns Hopkins University-School of Medicine
Baltimore, Maryland 21218

Johns Hopkins University is an equal opportunity employer M/F/D/V.