PhD student in Laboratory of Molecular Pathogenetics

Ph.D. student will work on innovative projects regarding different ways in which epigenetic signals and transcription factor networks govern self-renewal, cell specification, differentiation, and reprogramming – a crucial step for advances in cell-based therapy and perspectives for medicine.

Using mouse models, we aim to investigate how disruption of transcription networks affect epigenetic landscapes and downstream targets during embryonic development. We will use Cre/loxP and Crispr/Cas9 technologies, global gene expression profiling (RNA sequencing), single cell RNA sequencing, CUT&Tag chromatin profiling, FACS, confocal microscopy, immunohistochemistry, and in situ hybridization to evaluate embryonic phenotypes.

Who are we:

Using genetically modified mouse models and gene expression profiling, we identify molecular targets for the development of preventive and diagnostic strategies.

Our focus:

• transcriptional regulation in neurosensory development and maintenance in the inner ear model
• the molecular mechanisms involved in pathologies associated with diabetes mellitus
• the molecular causes of abnormal embryonic development

More information can be acquired from our websites:

https://www.ibt.cas.cz/en/research/laboratory-of-molecular-pathogenetics/

https://www.biocev.eu/en/research/development-of-diagnostic-and-therapeutic-procedures.7/molecular-pathogenetics.13

Related publications:

ISL1 is necessary for auditory neuron development and contributes towards tonotopic organization. Filova I, et al. accepted PNAS (2022).

Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification. Petitpré Ch, et al. Nature Communications 13, 3878 (2022).

Bohuslavova et al. NEUROD1 Is Required for the Early α and β Endocrine Differentiation in the Pancreas. Int J Mol Sci. 2021, 22(13):6713.

Filova I et al. Combined Atoh1 and Neurod1 Deletion Reveals Autonomous Growth of Auditory Nerve Fibers. Molecular Neurobiology. 2020, 57: 5307–5323.

Bohuslavova et al. NEUROD1 Is Required for the Early α and β Endocrine Differentiation in the Pancreas. Int J Mol Sci. 2021, 22(13):6713.

Bohuslavova R et al. HIF-1 is required for development of the sympathetic nervous system. PNAS, 2019 116 (27) 13414-13423.