PhD proposal at Université Paris Cité : Probing bioenergetics at single mitochondria by AFM-SECM microscopy

Organisation/Company

Université Paris Cité

Research Field

Chemistry » Biochemistry

Researcher Profile

First Stage Researcher (R1)

Country

France

Application Deadline

13 Jul 2024 – 23:30 (Europe/Paris)

Type of Contract

Temporary

Job Status

Full-time

Offer Starting Date

1 Oct 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

The goal of this doctoral project is to study bioenergetics activities at the scale of single organelles, the mitochondria, by using an ultra-sensitive electrochemical nano-imaging technique. 

Mitochondria are the “energy powerhouses” of living cells, synthesizing the chemical bioenergy fuel, ATP, under coupling with the activity of the enzymatic respiratory chain. Studying the efficiency of electron transport within the chain, via the oxidation of various coenzymes (NADH, quinone, cytochrome-c) with O2 as the final acceptor, provides information about the bioenergetics functioning of mitochondria in physio and pathological conditions. In this project, individual mitochondria will be functionally imaged, under different respiratory, redox and metabolic states, using the ultra-resolved and dual atomic force-electrochemical nanoscopy (AFMSECM). This imaging technique is currently the only one capable of constructing a topographical image of a single living mitochondrion while simultaneously mapping its redox reactivity, at an unprecedented sub-particle scale.

Keywords. Nano-bioelectrochemistry, AFM-SECM imaging, mitochondria, respiratory chain

Requirements

Research Field

Biological sciences

Education Level

Master Degree or equivalent

Languages

ENGLISH

Level

Good

Additional Information
Work Location(s)

Number of offers available

1

Company/Institute

Laboratory of Molecular Electrochemistry – Universite Paris Cité

Country

France

City

Paris

Postal Code

75013

Street

15, rue Jean-Antoine de Baif

Geofield

Where to apply

E-mail

[email protected]

Contact

City

PARIS

Website

https://u-paris.fr/

Street

85 boulevard Saint-Germain

Postal Code

75006

STATUS: EXPIRED