Molecular basis for differential susceptibility to stress in freshwater invertebrates, BBSRC SWBio DTP PhD studentship 2025

About:

The BBSRC-funded SWBio DTP involves a partnership of world-renown universities, research institutes and industry, based mainly across the South West and Wales.

This partnership has established international, national and regional scientific networks, and widely recognised research excellence and facilities.

We aim to provide you with outstanding interdisciplinary bioscience research training, underpinned by transformative technologies.

Programme Overview:

You will be recruited to a broad, interdisciplinary project, supported by a multidisciplinary supervisory team, with many cross-institutional projects available. There are also opportunities to:

• apply your research in an industrial setting (DTP CASE studentships).

• undertake research jointly with our core and associate partners (Standard DTP studentships with an associate partner).

• work with other national/international researchers.

• undertake fieldwork.

Our structured training programme will ensure you are well equipped as a bioscience researcher, supporting careers into academia, industry and beyond. 

First year

We provide a broad awareness of the fundamental research approaches in life sciences and how they could be applied to real-life situations through:

• two rotation projects – both allied with but in different disciplinary areas related to the PhD project.

• three taught units – training in Statistics, Bioinformatics, coding, experimental design, innovation and understanding the impact of your research.

Of note: You will need to successfully complete the first year to progress into your second year of studies. Also, if you are unable to continue your PhD, an MRes exit route is available upon successful completion of the first year.

Second to fourth years

The remaining years will be more like a conventional PhD, where you will focus on your PhD project.

Project Description

Living organisms are continuously exposed to environmental stressors that affect their health, survival and ability to reproduce. In some highly stressful environments however, such as heavily polluted ecosystems that would be expected to cause high mortality rates, populations are thriving. This phenomenon has been attributed for the most part to genetic adaptation, but in many cases the extend of genetic adaptation observed is insufficient to explain the level of stressor resistance seen. Epigenetic changes contributing to phenotypic plasticity and microbiome-extension of host adaptive phenotypic plasticity are two likely important, and interacting, contributing mechanisms, but remain poorly characterised. Addressing this knowledge gap will provide novel and fascinating insight into how organisms interact with their environment in order to overcome adverse conditions and result in important knowledge to understand the consequences of exposure to stressors in natural and anthropogenic environments. The applications of this knowledge are vast and range from improvement of the resilience and welfare of farmed animals, contributing to food security, to a better management of the sustainability of wild populations and preserving biodiversity.

This project will address the following questions: How do organisms cope with stressors in their environment? What are the molecular mechanisms employed to allow survival under stressful conditions? What are the temporal dynamics and broader consequences of the alterations seen?

The student will test the hypothesis that epigenetic variation and microbiome plasticity, in addition to genetic adaptation, contribute to stressor tolerance.

The student will use Daphnia pulex, a keystone small crustacean species in freshwaters, as a model system. We have already identified a number of natural Daphnia populations with extensive metal tolerance, which is partly heritable in clean conditions and partly due to plasticity. Metals are particularly interesting because they constitute one of the most common contaminants in freshwater systems while many metals are also essential elements within the body, and therefore they will be used as an exemplar stressor in this project. The student will be able to utilise this unique biological resource to investigate how their genome, epigenome and microbiome quantitatively account for metal tolerance and whether tolerance to a specific metal is developed at the expense of loss of fitness (growth; reproduction; survival; resistance to other stressors). 

The student will receive extensive training in state of the art techniques including, in vivo experimental techniques, genome, epigenome and microbiome sequencing and advanced bioinformatics analysis.