Modular DNA nanopores for synthetic biology

The aim of this project is to develop artificial cells that can programmably communicate. In biology, cells are miniature factories where different compartments handle specific tasks. Directed communication between molecules and reaction pathways in each zone ensures that tasks are undertaken efficiency, but current technologies cannot replicate this level of programmable information transfer. To develop sensing, catalysis, and tissue engineering technologies inspired by cellular systems, we need to engineer dynamic information highways between artificial compartments.

This project will use modular, responsive DNA nanostructures to form nanopores and channels in synthetic vesicles and droplets. Controlling the mixing and transfer of cargo within these networked systems will harness knowledge in nanotechnology and self-assembly to generate nanoreactors for chemical transformations. Engineering the migration of molecules across membrane boundaries will offer benefits in biotechnology and nanochemistry – for the triggered release of cargo, data transmission, and chemical fractionation and computation.

This project is a collaboration between the DNA nanotechnology (Dr. Rizzuto) and soft matter (A/Prof Wang) labs at UNSW. It involves cross-cutting investigations exploring DNA assembly in lipid nanoparticles, partnering with industry collaborators in the vaccine and therapy spaces. You’ll learn a range of chemical synthesis, self-assembly and microscopy techniques, converging on the fields of supramolecular chemistry, nanotechnology and bioengineering. The project is based on work recently published in Angewandte Chemie

and PNAS.

Check out our group websites here: rizzutogroup.org and annawanglab.com

Applying

Domestic or international students graduated or expecting to graduate with degrees in Chemistry, Biotechnology, Nanoscience or related disciplines are encouraged to apply.

If you meet the UNSW HDR admission requirements, please send an email directly to Dr Felix Rizzuto (f.rizzuto@unsw.edu.au) with the following information:

Email subject: Prospective PhD for DNA nanopores

Please provide:

–  Your background, experience, and research interests.

–  When you expect to be able to start your PhD if successful.

– A copy of your CV, along with undergraduate and any post-graduate academic transcripts.

– The names and contact information for 2 references.