Cardiovascular Physiologist: SRA – 118518

UCSD Layoff from Career Appointment: Apply by 09/19/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 09/29/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.

The Department of Medicine (DOM) is the largest department within the UCSD School of Medicine and is responsible for fulfilling the teaching, research and clinical missions of the University of California, San Diego School of Medicine. The department employs approximately 500 salaried faculty, 168 academic appointees, 331 residents, fellows and postdocs and 480 staff personnel.

The cardiovascular research laboratory in the Department of Medicine at the University of California, San Diego, seeks a Staff Research Associate 3 (SRA3) who is highly motivated, creative, and devoted to the project goals. One position in cardiovascular physiology is immediately available. Our research focuses on endothelial dysfunction in the heart and lung during cardiovascular diseases. The incumbent will design and implement research protocols and conduct experiments for ongoing research projects in the laboratory. Duties and Responsibilities are 1) perform physiological experiments in mice, including evaluation of cardiac function using Millar system (e.g., RVSP, LVSP, dP/dt, EF), measurement of pulmonary and coronary flow using an ultrasound system, and isometric tension measurements in mouse distal pulmonary and coronary arteries using a wire myograph, 2) conducting molecular biological experiments (e.g., Western Blot, immunoprecipitation, PCR, qPCR, gene cloning), 3) electrophysiological experiments to measure Ca2+ and ROS concentration in cytoplasm and mitochondria and assess GAP junction intercellular communication, 4) immunofluorescence experiment, and 5) isolation of vascular endothelial/smooth muscle cells from mouse lungs and hearts. The incumbent will perform data acquisition and analysis, grant and manuscript preparation, and presentation of findings at weekly lab meetings. Additional responsibilities include maintaining transgenic mice colonies (i.e., breeding and genotyping), managing lab research records (e.g., methodological approaches, experimental records, and protocols), and maintaining a clean lab and the function of lab equipment. Aseptic techniques for tissue culture and animal handling are mandatory skills in our laboratory.

• Theoretical and working knowledge in advanced molecular biology and cell physiology.

• Aseptic techniques for cell culture.

• Demonstrated ability to contribute to experimental design, analysis, interpretation of the results, and troubleshooting.

• Demonstrated experience assisting with writing scientific manuscripts and contributing to teamwork in molecular biological experiments.

• Demonstrated ability and experience to isolate heart and lung vascular endothelial and smooth muscle cells and prepare primary cultured cells for molecular biological and cell physiological experiments.

• Demonstrated ability and experience in gene cloning and silencing, production of Adeno- and Adeno-associated virus to transduce genes in the cells and tissues.

• Demonstrated ability and experience in Western Blot, immunoprecipitation, site-directed mutagenesis, PCR, qPCR, and single-cell RT-PCR.

• Demonstrated ability and experience in electrophysiological experiments and immunofluorescence experiments using a fluorescence microscope.

• Demonstrated skill to work with animals, as well as animal and human tissues.

• Demonstrated knowledge of the following specialized programs; SnapGene, GraphPad, EndNote, and MS Word/Excel/PowerPoint.

• Demonstrated ability to read scientific literature, understand appropriate study design, follow study procedures and protocol, and maintain participant confidentiality.

• Demonstrated effective time management skills to plan and organize work assignments in an efficient and productive manner.

• Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.

• Effective and diplomatic oral and written communication and interpersonal skills. Ability to work with a variety of personnel from different laboratories and instruct in optimal procedures for assays.

• Employment is subject to a criminal background check.

• Must be available to work some weekends & evenings to complete experiments.

• Must be able to work with laboratory animals (mice).

Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.

UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational – or “bench-to-bedside” – research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

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